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AL0103000

Sigma-Aldrich

ALiCE® Cell Free Protein Expression Kit (Plant Cell Lysate)

An eukaryotic cell free expression system with unprecedented 3 mg/mL recombinant protein yield in batch mode and post-translational modifications enabled

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About This Item

Code UNSPSC :
41105500

Forme

liquid (aqueous solutions)

Conditionnement

pkg of 6 × 200 μL (AL0103200 (ALiCE® Midi Kit))
pkg of 6 × 50 μL (AL0103050 (ALiCE® Mini Kit))
pkg of 6 × 500 μL (AL0103500 (ALiCE® Maxi Kit))

Technique(s)

protein expression: suitable

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

Cell-free protein expression (CFPE) systems derived from crude cell extracts have been used for decades as a research tool and have introduced many attractive advantages to protein expression technology. However, a critical barrier to the adoption of CFPE systems as alternatives to cell-based approaches has been the problem of relatively low protein yields.

To address this problem, ALiCE® a novel eukaryotic cell-free expression system was created by taking advantage of plant cell biology. ALiCE® offers unprecedented yields of up to 3 mg/mL of protein in batch mode. As such, it enables you to overcome the limitations of living host systems and to obtain “difficult to produce” proteins in a matter of hours instead of weeks.

ALiCE® utilizes tobacco cell lysates, depleted of vacuoles. The system contains all factors required for in vitro transcription (RNA polymerases, NTPs) and translation reactions (ribosomes, translation initiation/elongation factors, tRNA, etc.). Using a simple protocol, both the RNA transcription and translation take place in a single tube (coupled transcription/translation).

Reactions are started by simply mixing plasmid DNA with the ALiCE® Reaction mix. The outstanding protein yields of ALiCE® are based on highly efficient protein expression over the duration of the reaction. Consequently, we recommend a reaction duration of 48 h.

ALiCE® is provided with two expression vectors, pALiCE01 and pALiCE02, which are used according to the targeting of the protein of interest. These have been specially tailored to offer excellent protein yield when used with the ALiCE® expression system. pALiCE01 is used for expression in ALiCE® cytosolic fraction. Targeting the microsomes with pALiCE02 offers the opportunity to express protein with post-translational modifications like disulfide bonds.

Application

Besides screening the ALiCE® system can also be used for larger scale expression of target proteins. Potential additional applications of the ALiCE® system include:

  • Target protein characterization
  • Protein optimization
  • Mutant screening
  • Protein-protein interactions
  • Expression analysis
  • Protein localization analysis
  • Protein structure analysis
  • Post-translational modification assays
  • Metabolomics
  • Herbicide screening

Caractéristiques et avantages

ALiCE® Cell Free Protein Expression Kit utilizes cell lysate derived from plant cells to produce recombinant protein at 3 mg/mL in batch mode.
  • High-yield: Up to 3 mg/mL expressed protein
  • Easy to use: One-step reaction protocol
  • Fast: High yield in just 48 hours
  • Flexible: Express “difficult to produce” proteins, cytotoxic proteins, etc.

Composants

ALiCE® Mini Kit - AL0103050 Comprises
ALiCE® Lysate, 6 x 50 μL
Vector pALiCE01, 15 μL
Vector pALiCE02, 15 μL
ALiCE® Tube Cap, 0.6 mm hole

ALiCE® Midi Kit - AL0103200 Comprises
ALiCE® Lysate, 6 x 200 μL
Vector pALiCE01, 50 μL
Vector pALiCE02, 50 μL
ALiCE® Tube Set, 12 pcs

ALiCE® Maxi Kit - AL0103500 Comprises
ALiCE® Lysate, 6 x 500 μL
Vector pALiCE01, 50 μL
Vector pALiCE02, 50 μL
ALiCE® Tube Set, 12 pcs

Stockage et stabilité

  • ALiCE® Reaction (Lysate) mix: store at -70 °C
  • Vector pALiCE01: store at -20 °C to -70 °C
  • Vector pALiCE02: store at -20 °C to -70 °C
  • ALiCE® Tube caps/Tube set: store at room temperature

Informations légales

ALiCE is a registered trademark of LenioBio GmbH

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Certificats d'analyse (COA)

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Articles

Cell-Free Protein Synthesis or Expression

A form of synthetic biology, cell-free protein synthesis or expression (CFPE) is a rapid method to produce recombinant proteins in solution without involving cells. Using biomolecular translation machinery extracted from a range of different cell types, researchers can express a protein of interest within a day.

Protocoles

ALiCE®Cell-Free Protein Synthesis System Protocol

Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.

AliCE® Cell-Free Protein Synthesis Kit Quick Start Guide

Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.

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