A9891

7-Amino-4-methylcoumarin

Chromophore for substrates

• Synonyme(s) : Coumarin 120
• Formule empirique (notation de Hill): C₁₀H₉NO₂
• Numéro CAS: 26093-31-2
• Poids moléculaire : 175.18
• Numéro Beilstein : 142231
• Numéro CE : 247-454-8
• Numéro MDL: MFCD00006868
• Code UNSPSC : 12352204
• ID de substance PubChem : 24891406
• Nomenclature NACRES : NA.32

Propriétés

• Niveau de qualité: 200
• Description: chromophore for enzyme substrates
• Pureté: ≥98% (HPLC)
• Forme: powder
• Pf: 223-226 °C (lit.)
• Solubilité: acetone: 10 mg/mL, clear, colorless to yellow
• Fluorescence: λ_ex 365 nm; λ_em 440 nm in ethanol(lit.)
• Température de stockage: 2-8°C
• Chaîne SMILES: CC1=CC(=O)Oc2cc(N)ccc12
• InChI: 1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3
• Clé InChI: GLNDAGDHSLMOKX-UHFFFAOYSA-N

Description

Application

7-Amino-4-methylcoumarin has been used:

• to determine cathepsin B like- activity
• as a substrate for leucine aminopeptidase (LAP)
• in chymotryptic assay
• as a standard to detect the activation of caspase-3 during
• sanguinarine-induced damage
• protection by human DEAD-box DDX3

Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.

Actions biochimiques/physiologiques

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

Notes préparatoires

Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).

Autres remarques

Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves