A9207
Avidin−Agarose from egg white
aqueous glycerol suspension
Synonyme(s) :
Avidin agarose
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About This Item
Produits recommandés
Source biologique
egg white
Niveau de qualité
Conjugué
agarose conjugate
Forme
aqueous glycerol suspension
Matrice
cross-linked 6% beaded agarose
Activation de la matrice
epichlorohydrin
Fixation de matrice
amino
Espaceur de matrice
15 atoms
Température de stockage
−20°C
Description générale
Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.
Application
Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
- in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
- in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
- in avidin bead assay for purification of labelled biotin
- for capture of glycoproteins from murine cornea samples
Actions biochimiques/physiologiques
Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.
Définition de l'unité
One unit will bind 1.0 μg of d-biotin.
Forme physique
Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Contenu apparenté
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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