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A8103

Sigma-Aldrich

Anti-hABH1 antibody, Mouse monoclonal

clone hABH1-151, purified from hybidoma cell culture

Synonyme(s) :

Anti-Human AlkB Homologue 1, Monoclonal Anti-hABH1 antibody produced in mouse

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About This Item

Numéro MDL:
MFCD06412250
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

hABH1-151, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~45 kDa

Espèces réactives

human, monkey

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of 293T cells

Isotype

IgG1

Numéro d'accès UniProt

Q13686

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ALKBH1(8846)

Description générale

Anti-hABH1 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH1-151 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human hABH1, conjugated to keyhole limpet hemocyanin (KLH).
Human AlkB homologue 1 (hABH1) is one of the eight homologs of E. coli AlkB. Homology modeling studies have analyzed the role of hABH1 gene in DNA repair after alkaylation-induced damage.

Spécificité

Monoclonal Anti-hABH1 (human AlkB homologue) recognizes human and monkey ABH1. It does not recognize hABH2 or hABH3. The antibody epitope resides within amino acids 19-34 of human hABH1.

Application

Anti-hABH1 antibody, Mouse monoclonal has been used in
  • chromatin immunoprecipitation(ChIP)
  • immunohistochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation

Actions biochimiques/physiologiques

The human homolog, AlkB homolog 1, histone H2A dioxygenase (Alkbh1) is linked with several enzymatic actions, such as, DNA, mRNA or tRNA hydroxylation, cleavage at abasic (AP) site in DNA and demethylation of histones. It plays a key role in epigenetic gene silencing, as it is known to exhibit m6A demethylation activity . ALKBH1 participates in normal cell proliferation.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Role of ALKBH1 in the core transcriptional network of embryonic stem cells
Ougland R, et al.
Cellular Physiology and Biochemistry, 38(1), 173-184 (2016)
Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells
Muller TA, et al.
Biochemical and biophysical research communications, 495(1), 98-103 (2018)
Shankaracharya et al.
Interdisciplinary sciences, computational life sciences, 3(3), 175-181 (2011-10-01)
Inhibition of DNA repair mechanism through alkylating agents in tumor cells is an important method for cancer treatment. Alkylation damage repair gene AlkB was first reported in E. coli. In human and other mammals eight distinguishing homologs of AlkB were
Marianne Pedersen Westbye et al.
The Journal of biological chemistry, 283(36), 25046-25056 (2008-07-08)
The Escherichia coli AlkB protein and human homologs hABH2 and hABH3 are 2-oxoglutarate (2OG)/Fe(II)-dependent DNA/RNA demethylases that repair 1-methyladenine and 3-methylcytosine residues. Surprisingly, hABH1, which displays the strongest homology to AlkB, failed to show repair activity in two independent studies.
Rune Ougland et al.
Stem cells (Dayton, Ohio), 30(12), 2672-2682 (2012-09-11)
AlkB homolog 1 (ALKBH1) is one of nine members of the family of mammalian AlkB homologs. Most Alkbh1(-/-) mice die during embryonic development, and survivors are characterized by defects in tissues originating from the ectodermal lineage. In this study, we
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