Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

A6066

Sigma-Aldrich

Anti-Rat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonyme(s) :

Rabbit Anti-Rat IgG (whole molecule)–AP

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

rabbit

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous glycerol solution

Technique(s)

direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
western blot: 1:30,000-1:60,000

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

An immunoglobulin has two heavy chains and two lights chain connected by disulfide bond. It mainly helps in immune defense. IgG is a glycoprotein and is the major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs have four subclasses, namely, IgG1, IgG2a, IgG2b, and IgG2c. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgG is a useful tool for the analysis of target proteins.
The affinity isolated antibody is obtained from anti-rat IgG antiserum by immunospecific purification, which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to rat IgG. Anti-Rat IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde
Identity and purity of the antibody is established by immunoelectrophoresis (IEP) prior to conjugation. Electrophoresis of the product followed by diffusion versus anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation.

Spécificité

Binds all rat Igs. Anti-Rat IgG (whole molecule)-Alkaline Phosphatase antibody binds to all rat immunoglobulins.

Immunogène

Purified rat IgG

Application

Anti-Rat IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit has been used in:
  • western blot analysis
  • indirect enzyme-linked immunosorbent assay (ELISA)
  • RNA immunoprecipitation assay

Actions biochimiques/physiologiques

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Forme physique

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ivo Laidmäe et al.
Journal of biomedical materials research. Part A, 93(1), 29-36 (2009-06-02)
Fibrin glues (FG) of human or bovine origin are widely used for haemostasis and wound healing. In addition FGs are studied in many biomedical areas like cell therapy or tissue engineering. As any mammalian plasma products FG-s pose risk of
A beetle flight muscle displays leg muscle microstructure
Shimomura T, et al.
Biophysical Journal, 111(6), 1295-1303 (2016)
Characterization of the biological effect of fish fibrin glue in experiments on rats: Immunological and coagulation studies
Laidmae I et al
Journal of Biomedical Materials Research Part A, 93A, 29-36 (2012)
The Laboratory Rat (1998)
Molecular Genetics of Immunoglobulin (1987)

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique