A2765

Azocasein

protease substrate, chromogenic, powder

• Synonyme(s) : Sulfanilamide-azocasein
• Numéro CAS: 102110-74-7
• Numéro MDL: MFCD00130592
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• product name: Azocasein, protease substrate
• Source biologique: bovine
• Forme: powder
• Technique(s): ligand binding assay: suitable
• Solubilité: water: 5 mg/mL, clear, orange to very deep orange
• ε (coefficient d'extinction): ≥25 at 440 nm in 0.1 M NaOH at 1%
• Température de stockage: 2-8°C

Description

Description générale

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Application

Azocasein has been used as a substrate for determination of protease activity.

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Liaison

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)