A276
Monoclonal Anti-Na+/K+ ATPase (α Subunit) antibody produced in mouse
clone M7-PB-E9, ascites fluid
Synonyme(s) :
Anti-CMT2DD, Anti-HOMGSMR2
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
M7-PB-E9, monoclonal
Poids mol.
antigen ~110 kDa
Espèces réactives
canine, chicken, human, sheep, pig, bovine, mouse
Ne doit pas réagir avec
rat, Xenopus
Technique(s)
immunocytochemistry: suitable
immunofluorescence: 1:20
immunohistochemistry (frozen sections): 1:100
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:500-1:5000
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ATP1A1(476)
Description générale
The Na+/K+ ATPase (NKA) enzyme contains α, β and γ subunits. This enzyme is widely expressed. ATPase Na+/K+ transporting subunit α 1 (ATP1A1) is a principal catalytic subunit of NKA. It has a molecular mass of about 110kDa with 10 transmembrane segments. α-subunit has four different isoforms with tissue specific expression. α1 is primarily expressed in various tissues, including kidney, nerves and lung. While α2 is expressed in skeletal muscle and heart, α3 is found in the brain and α4 is apparently localized to testis and specifically to spermatozoa. ATP1A1 is encoded by the gene mapped to human chromosome 1p13-p11.
Immunogène
sheep kidney α subunit of Na+/K+ ATPase.
Application
Monoclonal Anti- Na+/K+ ATPase ( α Subunit) antibody produced in mouse has been used in flow cytometry.
Actions biochimiques/physiologiques
The Na+/K+ ATPase is implicated in the maintenance of ion homeostasis for regular neuronal excitability, secondary transport and neurotransmitter uptake. ATPase Na+/K+ transporting subunit α 1 (ATP1A1) participates in both pumping and signaling functions. ATP1A1 regulates cellular transmembrane sodium and potassium ion gradients essential for the control of resting membrane potential. Additionally, nucleotide binding domain of ATP1A1 facilitates cellular signal transduction and cell growth. Deficiency of the gene leads to development of major depressive disorder (MDD).
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the α subunit, and an associated smaller glycoprotein, termed the β subunit. Studies indicate that there are three isoforms of the α subunit (α 1, α 2, α 3) and two isoforms of the β subunit (β 1 and β 2) encoded by two multigene families.
The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The α 1 and β mRNAs are present in all cell types examined, whereas the α 2 and α 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The α subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.
The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The α 1 and β mRNAs are present in all cell types examined, whereas the α 2 and α 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The α subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.
Description de la cible
Na+/K+ transporting ATPase subunit α1 is an ion transport pump critical for maintaining the gradient of Na and K ions across the plasma membrane.
Forme physique
solution containing 1 mg/ml BSA and 0.05% sodium azide
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
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