A1935

Photobiotin acetate salt

For labeling DNA probes for hybridizations

• Synonyme(s) : Biotin {3-[3-(4-azido-2-nitroanilino)-N-methylpropylamino]propylamide} acetate salt, N-(4-Azido-2-nitrophenyl)-N′-(3-biotinylaminopropyl)-N′-methyl-1,3-propanediamine acetate salt
• Formule empirique (notation de Hill): C₂₃H₃₅N₉O₄S · C₂H₄O₂
• Numéro CAS: 96087-38-6
• Poids moléculaire : 593.70
• Numéro MDL: MFCD00210085
• Code UNSPSC : 12352200
• ID de substance PubChem : 24890604
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Pureté: ≥95%
• Forme: film or powder
• Solubilité: H₂O: 10 mg/mL (Stable for at least 5 months if kept frozen and protected from light.)
• Température de stockage: −20°C
• Chaîne SMILES: CC(O)=O.CN(CCCNC(=O)CCCC[C@@H]1SCC2NC(=O)NC12)CCCNc3ccc(cc3[N+]([O-])=O)N=[N+]=[N-]
• InChI: 1S/C23H35N9O4S.C2H4O2/c1-31(12-4-10-25-17-9-8-16(29-30-24)14-19(17)32(35)36)13-5-11-26-21(33)7-3-2-6-20-22-18(15-37-20)27-23(34)28-22;1-2(3)4/h8-9,14,18,20,22,25H,2-7,10-13,15H2,1H3,(H,26,33)(H2,27,28,34);1H3,(H,3,4)/t18-,20-,22-;/m0./s1
• Clé InChI: FFBLNTOMOSLSQM-AYEYRVMASA-N

Description

Description générale

Photobiotin acetate is made up of biotin, which is bound with the help of a charged linker arm to a photoreactive arylazide group.

Application

Photobiotin acetate salt has been used to label DNA probes to perform DNA−DNA hybridization to determine genomic relatedness.

Actions biochimiques/physiologiques

Photobiotin acetate is capable of labeling single stranded DNA and linear or supercoiled double-stranded DNA.

Photobiotin is a photo-activatable analog of biotin used primarily for labeling DNA, RNA and proteins. Probes prepared using photobiotin can detect target DNA as little as 0.5 pg.

Attention

Light sensitive.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)