94149
Atto 647N-Streptavidin
BioReagent, suitable for fluorescence
Synonyme(s) :
Streptavidin-Atto 647N
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About This Item
Code UNSPSC :
12161900
Nomenclature NACRES :
NA.25
Produits recommandés
Gamme de produits
BioReagent
Niveau de qualité
Forme
powder
Fabricant/nom de marque
ATTO-TEC GmbH
λ
in ethanol (with 0.1% trifluoroacetic acid)
Absorption UV
λ: 650.0-656.0 nm Amax
Adéquation
corresponds for gel electrophoresis
suitable for fluorescence
Température de stockage
−20°C
Description générale
Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.
Application
Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.
Caractéristiques et avantages
Atto 647N- Streptavidin has the following benefits-
- Highly photostable. Strong absorption.
- Excellent fluorescent quantum yield.
- Highly resistant to ozone degradation.
- Minimal triplet formation.
Remarque sur l'analyse
dye-to-protein ratio ≥1.5
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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