83330

Pyruvate Kinase from rabbit muscle

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

• Synonyme(s) : ATP:pyruvate 2-O-phosphotransferase, PK
• Numéro CAS: 9001-59-6
• Numéro de classification (Commission des enzymes): 2.7.1.40 (BRENDA, IUBMB)
• Numéro CE : 232-616-2
• Numéro MDL: MFCD00081946
• Code UNSPSC : 12352204
• eCl@ss : 32160410
• Nomenclature NACRES : NA.54

Propriétés

• Forme: suspension
• Qualité: crystalline suspension in 3.8 M ammonium sulfate solution
• Activité spécifique: ~350 U/mg protein (10-40 mg/ml)
• Poids mol.: M_r ~230000
• pH: ~6
• Activité étrangère: lactate dehydrogenase ≤0.01%
• Température de stockage: 2-8°C

Description

Actions biochimiques/physiologiques

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA₂₈₀ = 0.54 for 1 mg(p)/ml, 1 cm path
Reported K_M values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.

Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

Définition de l'unité

1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)