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51412C

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Milieu essentiel minimum d′Eagle

with Earle′s Balanced Salts, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

MEM

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Description

for research or for further manufacturing use

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | mammalian: suitable
cell culture | stem cell: suitable

Composants

L-glutamine: no
NaHCO3: 2200 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Minimum Essential Medium (MEM) with Earle′s Balanced Salts was developed by Harry Eagle as a result of his studies to determine the essential nutritional requirements of mouse L cells and HeLa cells in culture. He demonstrated that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM is a modification of Eagle′s earlier medium Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. This medium has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle′s Balanced Salts, which makes it suitable for use in atmospheres charged with CO2 gas.

Application

Minimum essential medium (MEM) has been used as a growth medium for bone marrow mesenchymal stem cells (BMSCs) and neurons.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

Certificats d'analyse (COA)

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A novel pulsed STED microscopy method using FastFLIM and the phasor plots.
Sun Y, et al.
Proceedings of SPIE (2017)
Involvement of microRNA-23b in TNF-?-reduced BMSC osteogenic differentiation via targeting runx2.
Deng L, et al.
Journal of Bone and Mineral Metabolism, 1-13 (2017)
Debora Soncini et al.
Blood advances, 4(18), 4312-4326 (2020-09-12)
Metabolic reprogramming is emerging as a cancer vulnerability that could be therapeutically exploitable using different approaches, including amino acid depletion for those tumors that rely on exogenous amino acids for their maintenance. ʟ-Asparaginase (ASNase) has contributed to a significant improvement
Agata Nowacka et al.
Scientific reports, 10(1), 2054-2054 (2020-02-08)
PSD-95 is a major scaffolding protein of the post-synaptic density (PSD) of a glutamatergic synapse. PSD-95, via interactions with stargazin, anchors AMPA receptors at the synapse and regulates AMPAR currents. The expression of PSD-95 is regulated during synaptic plasticity. It
Dirk M Reiter et al.
PLoS pathogens, 7(8), e1002166-e1002166 (2011-08-11)
Many viruses attach to target cells by binding to cell-surface glycans. To gain a better understanding of strategies used by viruses to engage carbohydrate receptors, we determined the crystal structures of reovirus attachment protein σ1 in complex with α-2,3-sialyllactose, α-2,6-sialyllactose
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