506-05A

Human Lung Fibroblasts: HLF, adult

• Synonyme(s) : HLF, HLF cells
• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: human lung (normal parenchyma)
• Niveau de qualité: 100
• Conditionnement: pkg of 500,000 cells
• Fabricant/nom de marque: Cell Applications, Inc
• Mode de croissance: Adherent
• Caryotype: 2n = 46
• Morphologie: fibroblast
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): asthma; tuberculosis; cardiovascular diseases; cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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HLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.

HLF have been utilized in a number of research publications, for example to:

• Demonstrate differential gene expression patterns between fetal and adult lung fibroblasts, in particular showing that fetal fibroblasts express higher level of TGF-β receptor interacting protein 1 (TRIP-1) and consequently are less able to contract collagen than adult lung fibroblasts, leading to better healing of acute injuries in very immature lungs compared to higher levels of fibrosis observed in older patients (Navarro, 2009), and additionally discover that TRIP-1 regulates lung fibroblast/myofibroblast trans-differentiation (Nyp, 2014)
• Show that angiotensin II promotes lung fibrosis by activating expression of IL-4, IL-7, and PDGF-D increasing the extracellular matrix formation, and that this process can be blocked by angiotensin receptor inhibitor candesartan (Okada, 2009)
• Determine that phosphorylation of C/EBPβ contributes to lung fibrosis (Buck, 2011)
• Demonstrate that increased tissue rigidity activates toll-like receptor 2 and NF-κB in lung fibroblasts and induces expression of cytokines, CXCL1-3, IL-8 and TNF-α (Zheng, 2011)
• Demonstrate that 2-ethoxyestradiol, a non-estrogenic estradiol metabolite analog, inhibits cell growth and retards the progression of pulmonary fibrosis and hypertension (Tofovic, 2008, 2009)
• Identify the hypoxia-induced ROS/RhoA–ROCK dependent actin reorganization as a mediator of myofibroblast transformation and collagen synthesis in lung fibrosis (Ni, 2013)

Origine de la lignée cellulaire

Lungs

Application

pulmonary function, gene expression, healing and repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development

Composants

Basal Medium containing 10% FBS & 10% DMSO

Notes préparatoires

• 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 12 doublings

Procédure de repiquage

Please refer to the HLF Culture Protocol.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable