106-05F

Human Dermal Fibroblasts: HDF, fetal

• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: human neonatal foreskin or adult skin (normal)
• Niveau de qualité: 100
• Conditionnement: pkg of 500,000 cells
• Fabricant/nom de marque: Cell Applications, Inc
• Mode de croissance: Adherent
• Caryotype: 2n = 46
• Morphologie: Fibroblast
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

Origine de la lignée cellulaire

Skin

Application

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

Composants

Fibroblast Basal Medium containing 10% FBS & 10% DMSO

Notes préparatoires

• Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Procédure de repiquage

Please refer to the HDF Culture Protocol.

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable