ROAMYGL

Amyloglucosidase

from Aspergillus niger

• Synonyme(s) : Glucoamylase, disaccharidase-type-α-D-glucosidase
• Numéro de classification (Commission des enzymes): 3.2.1.3 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: Aspergillus niger
• Niveau de qualité: 100
• Forme: suspension
• Activité spécifique: ~14 units/mg protein (At 25 °C with glycogen as the substrate; standardized with BSA.)
• Poids mol.: M_r 97 kDa
• Conditionnement: pkg of 10 mL (10102857001 [100 mg])
• Fabricant/nom de marque: Roche
• Paramètres: 55 °C optimum reaction temp.
• pH optimal: 4.6-4.8
• Température de stockage: 2-8°C

Description

Description générale

Amyloglucosidase is synthesized by several Aspergillus genus species. It is a disaccharidase–type α-glucosidase. This enzyme is an exo-enzyme and one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Spécificité

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.
Heat inactivation: Heat inactivation is recommended at 80 °C for 45 minutes, followed by rapidly cooling down.

Application

Amyloglucosidase from Aspergillus niger can be used for the hydrolyzation of terminal α1,4- and α1,6-glucosidic bonds (glucose-glucose bonds) in polysaccharides (e.g., starch, dextrins, glycogen), removing glucose units sequentially from the non-reducing end of the molecule. The enzyme will also cleave maltose and maltosides (maltotriose, maltotetraose, etc.).

Actions biochimiques/physiologiques

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.

Définition de l'unité

Unit Conversion: One unit (+25 °C; glycogen as substrate) corresponds to 8.6 U (+60 °C; starch as substrate).

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash