PK-RO

Pyruvate Kinase (PK)

from rabbit muscle

• Synonyme(s) : PK
• Numéro de classification (Commission des enzymes): 2.7.1.40 (BRENDA, IUBMB)
• Code UNSPSC : 12352204

Propriétés

• Source biologique: rabbit muscle
• Niveau de qualité: 100
• Forme: suspension
• Activité spécifique: ~200 units/mg protein (at 25 °C (500 U/mg at 37 °C) with PEP as the substrate.)
• Conditionnement: pkg of 1 mL (10128155001 [10 mg]); pkg of 10 mL (10128163001 [100 mg])
• Fabricant/nom de marque: Roche
• pH optimal: 7.0-7.5
• Température de stockage: 2-8°C

Description

Description générale

ATP:pyruvate 2-O-phosphotransferase
Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn²⁺ and phosphoenolpyruvate.

Application

Pyruvate kinase has been used to measure ATPase activity and in the determination of adenylate concentration.

Actions biochimiques/physiologiques

Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. Apart from this, the enzyme exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.

Qualité

Contaminants: <0.001% GK, <0.002% “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each

Forme physique

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Notes préparatoires

Activator: PK requires Mg²⁺ (or Mn²⁺, Co²⁺) and K⁺ (or NH⁴⁺, Rb⁺) for full activity.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): No data available
• Point d'éclair (°C): No data available