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AGRMP-RO

Roche

Agarose MP

powder, pkg of 100 g (11388983001), pkg of 500 g (11388991001)

Synonyme(s) :

agarose

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About This Item

Code UNSPSC :
12352200

Source biologique

algae (seaweed)

Description

multipurpose agarose

Forme

powder

Conditionnement

pkg of 100 g (11388983001)
pkg of 500 g (11388991001)

Fabricant/nom de marque

Roche

Impuretés

0.090 sulphate

Couleur

white

Pf

38 °C

Solubilité

water: 10 g/L at 80 °C

Température de stockage

15-25°C
20-25°C

Chaîne SMILES 

Canonical SMILES: C1C2C(C(O1)C(C(O2)O)O)OC3C(C(C(C(O3)CO)O)OC4C(C5C(C(O4)CO5)OC6C(C(C(C(O6)CO)O)O)O)O)OIsomeric SMILES: C1[C@H]2[C@H]([C@@H](O1)[C@@H]([C@@H](O2)O)O)O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O[C@H]4[C@H]([C@H]5[C@@H]([C@@H](O4)CO5)O[C@H]6[C@@H]([C@H]([C@H]([C@H](O6)CO)O)O)O)O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

Clé InChI

MJQHZNBUODTQTK-WKGBVCLCSA-N

Description générale

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

Application

Agarose MP, a multi purpose agarose, is developed for analytical and preparative electrophoresis of nucleic acids and separation of high molecular weight DNA via pulse field gel electrophoresis (PFGE).It is also used for preparative electrophoresis and quantitative recovery of biologically active DNA. Agarose MP has also been used along with other supplements to store embryos in different developmental stages.

Caractéristiques et avantages

  • High Gel Strength: Agarose MP gels exhibit great strength, even at low concentrations. This ensures sturdy gels for robust performance in electrophoresis.
  • Versatile Applications: Agarose MP aids in the analysis of various samples, including high molecular weight molecules.
  • Rapid Migration without restriction: Even at low concentrations agarose MP gels facilitate the rapid migration of large DNA molecules without significant restriction, resulting in efficient separation.
  • Pulsed-Field Gel Electrophoresis (PFGE) Compatibility: Agarose MP is ideal for PFGE as it enables short separation times for large DNA molecules while maintaining the high resolution required for effective analysis.

Qualité

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Autres remarques

Agarose MP can produce high strength gels (e.g., the gel strength of a 1% agarose MP gel is ?1800 g/cm2). Even gels that have low concentrations of agarose MP are strong enough to be used for electrophoresis. The agarose matrix of such low concentration gels allows high molecular weight molecules to migrate rapidly without significant restriction.
Agarose MP is particularly well suited for pulsed-field gel electrophoresis, because it allows short separation times for large DNA molecules, while maintaining the high resolution required for effective analysis.
For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


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Les clients ont également consulté

Pei Yun Lee et al.
Journal of visualized experiments : JoVE, 62(62), 3923-3923 (2012-05-02)
Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose)
François Sipieter et al.
PloS one, 10(10), e0140924-e0140924 (2015-10-31)
Uncoupling of ERK1/2 phosphorylation from subcellular localization is essential towards the understanding of molecular mechanisms that control ERK1/2-mediated cell-fate decision. ERK1/2 non-catalytic functions and discoveries of new specific anchors responsible of the subcellular compartmentalization of ERK1/2 signaling pathway have been

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