11767305001
Roche
TUNEL Enzyme
from calf thymus recombinant in E. coli
Synonyme(s) :
transferase dUTP nick end labeling, tunel
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About This Item
Code UNSPSC :
41105600
Produits recommandés
Produit recombinant
expressed in E. coli
Niveau de qualité
Forme
solution
Utilisation
sufficient for 20 tests
Conditionnement
pkg of 2 × 50 μL
Fabricant/nom de marque
Roche
Température de stockage
−20°C
Description générale
TUNEL enzyme solution, containing terminal deoxynucleotidyl transferase from calf thymus recombinant in E. coli.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.
One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.
Application
The enzyme solution contains terminal deoxynucleotidyl transferase from calf thymus, recombinant in E. coli, needed to perform the TUNEL reaction for detecting apoptosis in situ.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.
The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.
Forme physique
Terminal deoxynucleotidyl transferase (TdT) in an optimized concentration, dissolved in reaction buffer. Ready-to-use with TUNEL Label.
Notes préparatoires
Working solution: TUNEL Enzyme is used in combination with TUNEL Label to prepare the TUNEL reaction mixture.
To produce a TUNEL solution sufficient for one test:
Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
To produce a TUNEL solution sufficient for one test:
- Mix 5μl TUNEL Enzyme with 45μl TUNEL Label prior to use.
- For negative control use 50μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45μl TUNEL Label with 5μl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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