11088939001

Tumor Necrosis Factor-α, human (hTNF-α)

recombinant (yeast)

• Synonyme(s) : hTNF, human necrosis factor
• Code UNSPSC : 12352207

Propriétés

• Source biologique: human
• Niveau de qualité: 100
• Produit recombinant: expressed in yeast
• Essai: ≥95% (SDS-PAGE)
• Forme: solution
• Puissance: ≤0.01 ng/mL EC₅₀
• Activité spécifique: >1*10^8 U/mg
• Poids mol.: 17,000 Da
• Conditionnement: pkg of 1,000,000 U (10 μg, 1ml)
• Fabricant/nom de marque: Roche
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: <0.1 EU/μgtested (LAL test)
• Numéro d'accès UniProt: Q9UBM5
• Température de stockage: −20°C
• Informations sur le gène: human ... TNF(7124)

Description

Description générale

>1 x 10^⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.

Spécificité

Human hTNF-α is effective on human and mouse cells.

Species specificity: human, mouse

Application

Tumor Necrosis Factor-α (hTNF-α)

• Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
• Has activating and growth stimulating activities on a variety of normal cells.
• Has antiviral activity on many cell types in vitro.
• Can cause tumor necrosis of certain tumors in vivo.

Actions biochimiques/physiologiques

Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.

Qualité

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)

Séquence

Chain Length 157 AA

One polypeptide chain (157 amino acids), identical to natural hTNF-α.

Définition de l'unité

EC₅₀ definition: The amount of hTNF-α that is required to mediate half-maximal cytotoxicity (MTT cleavage) with WEHI 164 cells in the presence of actinomycin D (1 unit equals ≤0.01 ng/ml).

Forme physique

Solution, filtered through 0.2 μm pore size membrane.

Notes préparatoires

Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Autres remarques

For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash