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Key Documents

SCT023

Sigma-Aldrich

BioTracker 664 NIR Ca2+ AM Dye

Live cell imaging calcium (Ca2+) indicator for neuroscience and cancer applications.

Synonyme(s) :

Live cell imaging probe

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000

Technique(s)

cell based assay: suitable

Méthode de détection

fluorometric

Conditions d'expédition

ambient

Description générale

Calcium (Ca2+) play a vital role in the physiology and biochemistry of the cell. Calcium ions play an important role in signal transduction pathways, neurotransmitter release from neurons, contraction of all muscle cell types, and in fertilization. Extracellular calcium is also important for maintaining the potential difference across excitable cell membranes, as well as proper bone formation.

The BioTracker 664 NIR Ca2+ Dye is a live cell far-red fluorescent calcium indicator. The dye changes fluorescent intensity greatly (1000-fold) when it binds to calcium. Multicolor imaging is possible between the dye and Hoechst, Fluorescein, Rhodamine, GFP, YFP and RFP etc., which are fluorescent probes or fluorescent proteins having fluorescent wavelength from UV region to visible area. The advantages of long wavelength region are greater tissue penetration and low phototoxicity.
BioTracker 664 NIR Ca2+ Dye can be introduced by microinjection, patch clamp and electroporation. BioTracker 664 NIR Ca2+ AM Dye is an acetoxymethyl ester version and can permeate cell membranes.

Spectral Properties
Absorbance: 650nm
Emission: 664nm

Application

Live cell fluorescent imaging
Research Category
Cell Imaging

Forme physique

Lyophilized

Stockage et stabilité

Store BioTracker 664 NIR Ca2+ AM Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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