SCR713M
TagGFP2 Simplicon RNA
to determine optimal transfection conditions to express the self-replicating RNA in cell lines
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352207
eCl@ss :
32161000
Nomenclature NACRES :
NA.51
Produits recommandés
Niveau de qualité
Technique(s)
nucleic acid detection: suitable (RNA)
Description générale
Simplicon is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome1. The Simplicon RNA contains only genes encoding the VEE RNA replication machinery while the structural proteins that are required to make an infectious particle have been completely removed and replaced with the transgene(s) of interest. Because Simplicon is a synthetic RNA, there is no risk of the transgene(s) integrating into the transfected cell’s genome. Moreover, the self-replicating nature of the Simplicon means that the target protein is continuously expressed in the presence of B18R protein and the selective agent, puromycin. The Simplicon technology has been successfully utilized for efficient human iPSC generation through the sustained expression of critical reprogramming factors, OKSG and OKSG-cMyc1.
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon TagGFP2 expresses an improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively.
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon TagGFP2 expresses an improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively.
Application
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells.
Composants
1. TagGFP2 RNA: (Part No. CS222751) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
2. B18R RNA: (Part No. CS224516) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
2. B18R RNA: (Part No. CS224516) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
Qualité
• Discrete RNA band on RNA gel of appropriate size.
• GFP-positive cells observed one day after transfection.
• 40-60% GFP-positive BJ human fibroblasts with FACS analysis.
• GFP-positive cells observed one day after transfection.
• 40-60% GFP-positive BJ human fibroblasts with FACS analysis.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
12 - Non Combustible Liquids
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique