S7821-M
CpGenome Universal Methylated DNA
Enzymatically methylated human male genomic DNA to be used as a methylation-positive control for gene methylation studies.
Synonyme(s) :
Enzymatically methylated human male genomic DNA, Methylation-positive control
About This Item
Produits recommandés
Niveau de qualité
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
CpGenome
Application(s)
genomic analysis
Description générale
Materials Provided:
Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.
Validation:
Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only after the DNA was methylated.
CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
Forme physique
Stockage et stabilité
Informations légales
Clause de non-responsabilité
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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