NA57
Anti-DNA-PK (Ab-2) Mouse mAb (18-2)
liquid, clone 18-2, Calbiochem®
Synonyme(s) :
Anti-DNA Dependent Protein Kinase
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
18-2, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
human
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
Isotype
IgG1
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... PRKDC(5591)
Description générale
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing the splenocytes with mouse myeloma SP2/0 cells (see application references). Recognizes the ~460 kDa DNA-PK protein.
Recognizes the ~470 kDa DNA-PK protein in A431, HeLa, and HL60 cells, normal tonsil, normal colon, and breast carcinoma tissue.
This Anti-DNA-PK (Ab-2) Mouse mAb (18-2) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of DNA-PK (Ab-2).
Immunogène
DNA-PK purified from HeLa cells
Epitope: within amino acids 1-2713 of DNA-PK
Human
Application
Frozen Sections (1:50-1:100)
Immunoblotting (1:250-1:500)
Immunofluorescence (1:50-1:100)
Immunoprecipitation (10 l/mg protein lysate)
Neutralization Studies (see comments)
Paraffin Sections (1:50-1:100, heat/pressure cooker pre-treatment required)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 10 mM PBS, 0.2% BSA, pH 7.4.
Reconstitution
For long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Remarque sur l'analyse
Positive Control
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue
A-431, HeLa, or HL-60 cells or normal tonsil, normal colon, or breast carcinoma tissue
Autres remarques
Kharbanda, S., et al. 1997. Nature386, 732.
McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.
Peterson, S.R., et al. 1997. J. Biol. Chem.272, 10227.
Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.
Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.
Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol.8, 153.
Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem.271, 8936.
Connelly, M.A., et al. 1996. Gene175, 271.
Danska, J.S., et al. 1996. Mol. Cell. Biol.16, 5507.
Han, Z., et al. 1996. J. Biol. Chem.271, 25035.
Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.
Song, Q., et al. 1996. EMBO J.15, 3238.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Blunt, T., et al. 1995. Cell80, 813.
Hartley, K.O., et al. 1995. Cell82, 849.
Kirchgessner, C.U., et al. 1995. Science267, 1178.
Lees-Miller, S.P., et al. 1995. Science267, 1183.
Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.
Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.
Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.
McConnell, K.R., et al. 1997 J. Immunol.158, 2083-2089.
Peterson, S.R., et al. 1997. J. Biol. Chem.272, 10227.
Anderson, C.W. and Carter, T.H. 1996. Curr. Top. Microbiol. Immunol.217, 91.
Blunt, T., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10285.
Carpenter, C.L. and Cantley, L.C. 1996. Curr. Opin. Cell. Biol.8, 153.
Chan, D.W. and Lees-Miller, S.P. 1996. J. Biol. Chem.271, 8936.
Connelly, M.A., et al. 1996. Gene175, 271.
Danska, J.S., et al. 1996. Mol. Cell. Biol.16, 5507.
Han, Z., et al. 1996. J. Biol. Chem.271, 25035.
Lees-Miller, S.P. 1996. Biochem. Cell. Biol.74, 503.
Song, Q., et al. 1996. EMBO J.15, 3238.
Teraoka, H., et al. 1996. FEBS Lett.393, 1.
Blunt, T., et al. 1995. Cell80, 813.
Hartley, K.O., et al. 1995. Cell82, 849.
Kirchgessner, C.U., et al. 1995. Science267, 1178.
Lees-Miller, S.P., et al. 1995. Science267, 1183.
Poltoratsky, V.P., et al. 1995. J. Immunol.155, 4529.
Anderson, C.W. 1993. Trends Biochem. Sci.18, 433.
Gottleib, T.M. and Jackson, S.P. 1993. Cell72, 131.
This antibody will inhibit (approximately 50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Clone 18-2. Antibody should be titrated for optimal results in individual systems.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Tewodros Mamo et al.
Biochemical and biophysical research communications, 486(2), 307-313 (2017-03-17)
Osteosarcoma survival rate has not improved over the past three decades, and the debilitating side effects of the surgical treatment suggest the need for alternative local control approaches. Radiotherapy is largely ineffective in osteosarcoma, indicating a potential role for radiosensitizers.
Xiaosheng Wu et al.
Journal of immunology (Baltimore, Md. : 1950), 174(2), 934-941 (2005-01-07)
Activation-induced cytidine deaminase (AID) is required for Ig class switch recombination, a process that introduces DNA double-strand breaks in B cells. We show in this study that AID associates with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) promoting cell survival
Benu Brata Das et al.
The EMBO journal, 28(23), 3667-3680 (2009-10-24)
Human tyrosyl-DNA phosphodiesterase (TDP1) hydrolyzes the phosphodiester bond at a DNA 3' end linked to a tyrosyl moiety. This type of linkage is found at stalled topoisomerase I (Top1)-DNA covalent complexes, and TDP1 has been implicated in the repair of
Stéphanie Solier et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(32), 12866-12872 (2012-07-04)
The "apoptotic ring" is characterized by the phosphorylation of histone H2AX at serine 139 (γ-H2AX) by DNA-dependent protein kinase (DNA-PK). The γ-H2AX apoptotic ring differs from the nuclear foci patterns observed in response to DNA-damaging agents. It contains phosphorylated DNA
Kristen E Hurov et al.
Genes & development, 24(17), 1939-1950 (2010-09-03)
In response to DNA damage, cells activate a complex signal transduction network called the DNA damage response (DDR). To enhance our current understanding of the DDR network, we performed a genome-wide RNAi screen to identify genes required for resistance to
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