MABT83

Anti-Aggrecan Antibody, clone 6B4

clone 6B4, from mouse

• Synonyme(s) : Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 6B4, monoclonal
• Espèces réactives: bovine
• Réactivité de l'espèce (prédite par homologie): human (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001126
• Numéro d'accès UniProt: P16112
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ACAN(176)

Description

Description générale

Aggrecan (CSPG1, CSPCP, or CSPGCP) is a chondroitin-sulfate proteoglycan belonging to the family of letican proteins. Aggrecan is a large globar structure containing multiple domains including carbohydrate-recognition domains; complement binding protein domains; immunoglobulin domains; proteoglycan tandem repeats; and in some cases, EGF-like domains. It can be degraded by aggrecanase. The N-terminus of aggrecan binds ECM hyaluronan in the presence of the linking protein, and forms complex aggregates, whereas the C-terminus binds a number of other ECM proteins including tenascins, glycolipids, and fibulins. Aggrecan is a major component of cartilage tissue and it enables this tissue to withstand compression by maintaining the viso-elastic properties of cartilage. Aggrecan may also facilitate neural maturation and plasticity by contributing to the formation of perineuronal nets in the developing CNS.

Immunogène

Ovalbumin-conjugated linear peptide corresponding to human Aggrecan.

Application

Anti-Aggrecan Antibody, clone 6B4 is an antibody against Aggrecan for use in Western Blotting & IHC.

Immunofluorescent Analysis: A representative lot was used by an independent laboratory in human femoral chondrocytes (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in human chartilage tissue (Williams, R., et al. (2010). PLoS One. 5(10):e13246.).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Qualité

Evaluated by Western Blot in A1D1 purified protein from bovine tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Aggrecan in 10 µg of purified protein from bovine tissue lysate.

Description de la cible

~60 kDa observed. Uniprot describes three isoforms at ~250 kDa, ~246 kDa, and ~239 kDa Aggrecan is degraded by aggrecanase into a ~60 kDa protein and other higher molecular weight products (Lemons, M. L. et al. (2001). J Neurosci. 21(13):4772-4781. Forsang, A. J. et al. (1995). Biochem J. 310(Pt 1):337-343.).

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
A1D1 purified protein from bovine tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable