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Key Documents

MABS499

Sigma-Aldrich

Anti-Phosphothreonine Antibody, clone RM102

clone RM102, from rabbit, purified by affinity chromatography

Synonyme(s) :

p-Thr, pthr, phospho-threonine

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

RM102, monoclonal

Produit purifié par

affinity chromatography

Espèces réactives

human

Réactivité de l'espèce (prédite par homologie)

all

Technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pThr)

Description générale

Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.

Immunogène

Mix of recombinant protein and peptide corresponding to All Phosphothreonine.

Application

Anti-Phosphothreonine, clone RM102 is an antibody against Phosphothreonine for use in WB, ICC, IH, IP.
Immunocytochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on Calyculin A/Okadaic Acid treated A431 cells
Immunohistochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on human colon cancer tissue
Immunoprecipitation Analysis: A representative lot of this antibody at 1:500 dilution immunoprecipitated phosphothreonine modified proteins from Calyculin A/Okadaic Acid treated A431 cell lysate

Qualité

Evaluated by Western Blotting on Calyculin A/Okadaic Acid treated HeLa cell lysate

Western Blotting Analysis: A 1:2000 dilution of this antibody detected phosphothreonine modification on Calyculin A/Okadaic Acid treated HeLa cell lysate

Description de la cible

N/A kDa observed

Autres remarques

Concentration: Please refer to lot specific datasheet.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Yukika Saga et al.
Genes to cells : devoted to molecular & cellular mechanisms, 24(6), 422-435 (2019-04-20)
Dictyostelium STATa is a homologue of metazoan signal transducers and activators of transcription (STATs) and is important for morphogenesis. STATa is activated by phosphorylation on Tyr702 when cells are exposed to extracellular cAMP. Although two tyrosine kinase-like (TKL) proteins, Pyk2

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