MABS1812M

Anti-Furin Antibody, clone 16B1.1

clone 16B1.1, from mouse

• Synonyme(s) : Anti-FUR, Anti-PACE, Anti-PCSK3, Anti-SPC1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 16B1.1, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_001276752
• Numéro d'accès UniProt: P09958
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FURIN(5045)

Description

Description générale

Furin (UniProt: P09958; also known as EC: 3.4.2.75, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme, PACE) is encoded by the FURIN (also known as FUR, PACE, PCSK3) gene (Gene ID: 5045) in human. Furin is an ubiquitously expressed enzyme with endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. It is single-pass type I membrane protein that shuttles between the trans-Golgi network and the cell surface. It is synthesized with a signal peptide of 26 amino acids and a propeptide region (aa 27-107) that inhibits its activity. The inhibition peptide plays the role of an intramolecular chaperone. It is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Propeptide cleavage is a prerequisite for exit of Furin molecules out of the endoplasmic reticulum. A second cleavage within the propeptide region occurs in the trans-Golgi network, followed by the release of the propeptide and the activation of Furin. Mature Furin has a luminal region (aa 108-705), a helical domain (aa 716-738), and a cytoplasmic region (aa 739-794). The cytoplasmic domain responsible for its trans-Golgi network localization and recycling from the cell surface.

Spécificité

Clone 16B1.1 detects human Furin and it targets an epitope within 177 amino acids from the C-terminal half.

Immunogène

GST/His-tagged recombinant fragment corresponding to 177 amino acids from the C-terminal half of human Furin.

Application

Anti-Furin, clone 16B1.1, Cat. No. MABS1812, is a mouse monoclonal antibody that detects Furin in human cells and has been tested for use in Western Blotting.

Qualité

Evaluated by Western Blotting in U-251 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Furin in 10 µg of U-251 cell lysate.

Description de la cible

~100 kDa observed; 86.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1