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MABN88

Sigma-Aldrich

Anti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12

clone N1/12, from mouse

Synonyme(s) :

Electroneutral potassium-chloride cotransporter 2, Erythroid K-Cl cotransporter 2, Neuronal K-Cl cotransporter, electroneural potassium-chloride cotransporter 2, solute carrier family 12, potassium-chloride transporter), member 5

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

N1/12, monoclonal

Espèces réactives

rat, human

Technique(s)

immunohistochemistry: suitable

Isotype

IgG2aκ

Numéro d'accès NCBI

NP_599190

Numéro d'accès UniProt

Q63633

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... SLC12A5(57468)
rat ... Slc12A5(171373)

Description générale

KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA′s inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.

Spécificité

Shown by an independent laboratory to react in human and mouse tissue. (UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior)
This antibody recognizes KCC2

Immunogène

Epitope: Unknown
Recombinant protein corresponding to rat KCC2.

Application

Detect K+/Cl-Cotransporter (KCC2) using this Anti-K+/Cl-Cotransporter (KCC2) Antibody, clone N1/12 validated for use in IH.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected KCC2 in rat hypothalamus tissue.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Qualité

Evaluated by Immunohistochemistry in rat cortex area A-3 tissue.

Immunohistochemistry Analysis: 1:300 dilution of this antibody detected KCC2 in rat cortex area A-3 tissue.

Description de la cible

126 kDa Calculated

Forme physique

Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Rat cortex area A-3 tissue

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Sana Al Awabdh et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 42(2), 166-182 (2021-11-24)
The K+-Cl- cotransporter KCC2, encoded by the Slc12a5 gene, is a neuron-specific chloride extruder that tunes the strength and polarity of GABAA receptor-mediated transmission. In addition to its canonical ion transport function, KCC2 also regulates spinogenesis and excitatory synaptic function
Vladimir Jovasevic et al.
Nature neuroscience, 18(9), 1265-1271 (2015-08-19)
Fear-inducing memories can be state dependent, meaning that they can best be retrieved if the brain states at encoding and retrieval are similar. Restricted access to such memories can present a risk for psychiatric disorders and hamper their treatment. To
Deepti Chugh et al.
PloS one, 10(7), e0132366-e0132366 (2015-07-16)
Synapsins are pre-synaptic vesicle-associated proteins linked to the pathogenesis of epilepsy through genetic association studies in humans. Deletion of synapsins causes an excitatory/inhibitory imbalance, exemplified by the epileptic phenotype of synapsin knockout mice. These mice develop handling-induced tonic-clonic seizures starting

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