MABN72

Anti-pan-MAGUK Antibody, clone K28/86

clone K28/86, from mouse

• Synonyme(s) : discs, large homolog 4 (Drosophila), discs large homolog 4, disks large homolog 4, Tax interaction protein 15, post-synaptic density protein 95, Postsynaptic density protein 95, Synapse-associated protein 90
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: K28/86, monoclonal
• Espèces réactives: rat
• Réactivité de l'espèce (prédite par homologie): human (immunogen homology), mouse (based on 100% sequence homology)
• Technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1λ
• Numéro d'accès NCBI: NP_001356
• Numéro d'accès UniProt: P78352
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... DLG4(1742)

Description

Description générale

The MAGUKs are membrane-associated guanylate kinase related (MAGUK) proteins that are defined by their inclusion of PDZ, SH3 and GUK domains. The GUK domain that they have is structurally very similar to that of the guanylate kinases, which converts ATP into ADP. However, it is known to be catalytically inactive as the P-Loop which normally binds ATP is absent. It is thought that the MAGUKs have adopted the GUK domain for their own purposes, primarily based on its ability to form protein-protein interactions with cytoskeleton proteins, microtubule/actin based machinery and molecules involved in signal transduction. In addition many of the MAGUK proteins also contain regions homologous of CaMKII, WW and L27 domains. Some of the members of the MAGUK family of proteins include DLG4 (Disks large homolog 4), DLG2, PSD95, MPP1 and other cytoskeleton-interacting proteins.

Spécificité

This antibody exhibits cross reactivity against recombinant Chapsyn-110, SAP97, and SAP102. (UC Davis/NIH NeuroMab Facility, http://neuromab.ucdavis.edu)

Immunogène

Recombinant protein corresponding to a fusion domain of human PSD95/SAP90 proteins.

Application

Anti-pan-MAGUK Antibody, clone K28/86 is an antibody against pan-MAGUK for use in IH, WB & IP.

Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected the MAGUK family of proteins in rat cerebellum and rat hypothalamus tissues.

Immunoprecipitation Analysis: A previous lot of this antibody was successfully used by an independent laboratory in IP. (Rasband, M., et al. (2002). The Journal of Cell Biology. 159(4):663–672.)

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Qualité

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected members of the MAGUK protein family on 10 µg of rat brain tissue lysate.

Description de la cible

~ 85-110 kDa observed

Forme physique

Format: Purified

Protein G

Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Rat brain tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable