MABN2648

Anti-CHD-8L Antibody, clone 1C10-1

• Synonyme(s) : Chromodomain-helicase-DNA-binding protein 8;EC:3.6.4.12;ATP-dependent helicase CHD8;Axis duplication inhibitor;Duplin
• Code UNSPSC : 12352203

Propriétés

• Source biologique: rat
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 1C10-1, monoclonal
• Poids mol.: calculated mol wt 290.85 kDa; observed mol wt ~290 kDa
• Produit purifié par: using protein G
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: N-terminal
• Numéro d'accès Protein ID: NP_963999
• Numéro d'accès UniProt: Q09XV5
• Conditions d'expédition: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Chd8(67772)

Description

Description générale

Chromodomain-helicase-DNA-binding protein 8 (UniProt: Q09XV5; also known as EC:3.6.4.12, CHD-8, ATP-dependent helicase CHD8, Axis duplication inhibitor, Duplin) is encoded by the Chd8 (also known as Kiaa1564) gene (Gene ID: 67772) in murine species. CHD8 is a DNA helicase that acts as a chromatin remodeling factor and regulates transcription. It is expressed predominantly from early- to mid-stage mouse embryogenesis and is detected throughout embryos from 7.5 to 9.5 dpc. It localizes to the promoter regions of several β-catenin responsive genes and acts as a negative regulator of Wnt signaling pathway by regulating β-catenin activity. CHD8 is also reported to suppress p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Homozygous deletion of Chd8 gene in mice is reported to result in early embryonic death. Reduced expression of Chd8 gene has been associated with abnormal activation of RE-1 silencing transcription factor (REST), which suppresses the transcription of many neuronal genes. Mice heterozygous for Chd8 mutations exhibit myelination defects and manifest autism spectrum disorder (ASD) with behavioral characteristics that include increased anxiety, repetitive behavior, and altered social behavior. They also exhibit myelination defects Two isoforms of CHD-8 have been reported that are produced by alternative splicing. They are described as a full-length CHD-8L and a CHD-8S that contains only the NH2-terminal chromodomain. (Ref.: Kawamura, A., et al. (2021). Cell Rep. 35(1);108932; Katayama, Y., et al. (2016). Nature. 537(7622); 675-679; Nishiyama, M., et al. (2009). Nat. Cell Biol. 11(2); 172-182).

Spécificité

Clone 1C10-1 is a rat monoclonal antibody that detects Chromodomain-helicase-DNA-binding protein 8 (CHD-8). It targets and epitope within the N-terminal region and is expected to react with isoforms 1 and 2.

Immunogène

GST-tagged recombinant fragment corresponding to the first 334 amino acids from the N-terminal region of mouse CHD-8L.

Application

Quality Control Testing

Evaluated by Western Blotting in E14 Mouse brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CHD8L in E14 Mouse brain tissue lysate.

Tested Applications

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected CHD-8L in Chromatin Immunoprecipitation applications (Katayama, Y., et al. (2016). Nature. 537(7622); 675-679; Kawamura, A., et al. (2021). Cell Rep. 35(1);108932).

Western Blotting Analysis: A representative lot detected CHD-8L in Western Blotting applications (Katayama, Y., et al. (2016). Nature. 537(7622); 675-679).

Immunoprecipitation Analysis: A representative lot detected CHD-8L in Immunoprecipitation applications (Katayama, Y., et al. (2016). Nature. 537(7622); 675-679).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CHD-8L, clone 1C10-1 , Cat. No. MABN2648, is a rat monoclonal antibody that detects CHD-8L and is tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.

Forme physique

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1