MABF2745

Anti-CD200R Antibody, clone OX-108

• Synonyme(s) : CD200 cell surface glycoprotein receptor, CD200R, CRTR2, Cell surface glycoprotein CD200 receptor 1, Cell surface glycoprotein OX2 receptor 1, MOX2R, OX2R, UNQ2522/PRO6015
• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: OX-108, monoclonal
• Poids mol.: calculated mol wt 36.60 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100
• Technique(s): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; inhibition assay: suitable; surface plasmon resonance (SPR): suitable
• Isotype: IgG1κ
• Séquence de l'épitope: Extracellular domain
• Numéro d'accès Protein ID: NP_620161
• Numéro d'accès UniProt: Q8TD46
• Température de stockage: -10 to -25°C
• Informations sur le gène: human ... CD200R1(131450)

Description

Spécificité

Clone OX-108 is a mouse monoclonal antibody that detects CD200R. It targets an epitope within the extracellular domain.

Immunogène

Full-length, recombinant human CD200R.

Application

Quality Control Testing

Evaluated by Flow Cytometry with human peripheral blood mononuclear cells (PBMCs).

Flow Cytometry Analysis: 1.0 µg from a representative lot detected CD200R in one million human peripheral blood mononuclear cells (PBMCs).

Tested Applications

Surface Plasmon Resonance: A representative lot detected CD200R in surface plasmon resonance application (Hatherley, D., et al. (2004). Eur J Immunol. 34(6):1688-94).

Immunohistochemistry Application: A representative lot detected CD200R in immunohistochemistry application (Koning, N., et al. (2009). J Neuropathol Exp Neurol. 68(2):159-67).

Inhibition: A representative lot blocked ligand-receptor interactions in protein microarray application (Letarte, M., et al. (2005). BMC Biochem.1:6:2).

Flow Cytometry Analysis: A representative lot detected CD200R in flow cytometry application (Wright, G.J., et al. (2003). J Immunol. 171(6):3034-46; Foster-Cuevas, M., et al. (2004). J Virol. 78(14):7667-76; Koning, N., et al. (2009). J Neuropathol Exp Neurol. 68(2):159-67).

Immunofluorescence Analysis: A representative lot detected CD200R in immunofluorescence application (Letarte, M., et al. (2005). BMC Biochem.1:6:2).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Cell surface glycoprotein CD200 receptor 1 (UniProt: Q8TD46; also known as CD200 cell surface glycoprotein receptor, Cell surface glycoprotein OX2 receptor 1) is encoded by the CD200R1 (also known as CD200R, CRTR2, MOX2R, OX2R, UNQ2522/PRO6015) gene (Gene ID: 131450) in human. CD200R is an inhibitory receptor that plays a crucial role in the regulation of immune responses. It is primarily involved in maintaining immune homeostasis by delivering inhibitory signals that downregulate inflammatory responses and prevent overactivation of the immune system. Upon binding with its ligand, CD200, CD200R transduces signals that inhibit the activation of macrophages, dendritic cells, and other immune cells, thereby promoting tolerance and preventing autoimmune reactions. CD200R is expressed on various immune cells, including macrophages, dendritic cells, neutrophils, and certain subsets of T cells and B cells. It is also found on some non-immune cells, such as neurons and endothelial cells. The expression of CD200R1 is generally upregulated in response to inflammatory stimuli and during certain stages of immune cell differentiation. CD200R is implicated in various autoimmune diseases, cancer, infectious and neurodegenerative diseases.

Forme physique

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable