MABF2103

Anti-FcRH5 Antibody, clone F25

clone F25, from mouse

• Synonyme(s) : Fc receptor-like protein 5, FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, Immune receptor translocation-associated protein 2, IRTA2, CD307e
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: F25, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): ELISA: suitable; flow cytometry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_112571.2
• Numéro d'accès UniProt: Q96RD9
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FCRL5(83416)

Description

Description générale

Fc receptor-like protein 5 (UniProt: Q96RD9; also known as FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, FcRH5, Immune receptor translocation-associated protein 2, IRTA2, CD307e) is encoded by the FCRL5 (also known as FCRH5, IRTA2, UNQ503/PRO820) gene (Gene ID: 83416) in human. FcRH5 is a single-pass type I membrane protein that is expressed in marginal zone B-cells, immunoblasts, tonsillar germinal center centrocytes and in the intraepithelial and interfollicular regions of the tonsil. It is involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. It is shown to regulate BCR signaling and binds aggregated IgG and engages several of its Ig-domains to bind IgG. It is shown to be overexpressed in many lymphoma cell lines and on hairy cell leukemia cells. Serum levels of soluble FcRH5 are reported to be elevated in patients with several types of B cell tumors. FcRH5 is synthesized with a signal peptide (aa 1-15), which is cleaved off in the mature form that has a long extracellular domain (aa 16-851), a short transmembrane domain (aa 852-872), and a cytoplasmic tail (aa 873-977) that contains immunoreceptor tyrosine-based inhibitor motifs (ITIM). (Ref.: Franco, A., et al. (2013). J. Immunol. 190(11); 5739-46; Ise, T., et al. (2007). Leukemia 21(1); 169-174).

Spécificité

Clone F25 detects human Fc receptor-like protein 5. It targets an epitope with in the extracellular domain.

Immunogène

Epitope: extracellular domain

Full-length FcRH5 expression plasmid followed by a boost with 293T cells transfected with FcRH5 plasmid.

Application

Anti-FcRH5, clone F25, Cat. No. MABF2103, is a mouse monoclonal antibody that detects Fc receptor-like protein 5 and has been tested for use in ELISA, Flow Cytometry, and Western Blotting.

Flow Cytometry Analysis: A representative lot detected FcRH5 in Flow Cytometry applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).

Western Blotting Analysis: A representative lot detected FcRH5 in Western Blotting applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).

ELISA Analysis: A representative lot detected FcRH5 in ELISA applications (Ise, T., et. al. (2005). Clin Cancer Res. 11(1):87-96).

Research Category
Inflammation & Immunology

Qualité

Evaluated by Flow Cytometry in SU-DHL-5 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected FcRH5 in one million SU-DHL-5 cells .

Description de la cible

106.44 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.