MABF2075

Anti-MR1 Antibody, clone 26.5

clone 26.5, from mouse

• Synonyme(s) : Major histocompatibility complex class I-related gene protein, MHC class I-related gene protein, Class I histocompatibility antigen-like protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 26.5, monoclonal
• Espèces réactives: rat, bovine, mouse, human
• Conditionnement: antibody small pack of 25 μL
• Technique(s): flow cytometry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001522.1
• Numéro d'accès UniProt: Q95460
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MR1(3140)

Description

Description générale

Major histocompatibility complex class I-related gene protein (UniProt: Q95460; also known as MHC class I-related gene protein, Class I histocompatibility antigen-like protein, MR1) is encoded by the MR1 gene (Gene ID: 3140) in human. MR1 is a single-pass membrane protein that is ubiquitous in its distribution and can heterodimerize with B2M. It serves as an antigen-presenting molecule specialized in presenting microbial vitamin B metabolites. It is involved in the development and expansion of a small population of T-cells expressing an invariant T-cell receptor alpha chain known as mucosal-associated invariant T-cells (MAIT). MAIT lymphocytes are preferentially located in the gut lamina propria and may be involved in monitoring commensal flora or serve as a distress signal. MR1-restricted T cells can discriminate between microbes in a TCR-dependent manner. However, these cells are shown to be severely reduced in circulation in subjects with chronic HIV-1 infection. MR1 is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. The mature form has an extracellular domain (aa 23-302), a transmembrane domain (aa-303-323), and a short cytoplasmic tail (aa 324-341). Its ligand binding region is localized to amino acids 23-201. Five different isoforms of MR1 have been described that are produced by alternative splicing. (Ref.: Young, MH et al., (2013). PLOS one 8(1); e53789; Leeansyah, E., et al. (2013). Blood. 121(7); 1124-1135).

Spécificité

Clone 26.5 specifically detects Major histocompatibility complex class I-related gene protein (MR1). It targets an epitope with in the extracellular domain.

Immunogène

Epitope: extracellular domain

GST-tagged recombinant fragment corresponding to 280 amino acids from the extracellular domain of human MHC class I-related gene protein.

Application

Anti-MR1, clone 26.5, Cat. No. MABF2075, is a mouse monoclonal antibody that detects MHC class I-related gene protein and has been tested for use in Flow Cytometry, Function Analysis, and Immunoprecipitation.

Immunoprecipitation Analysis: A representative lot immunoprecipitated MR1 in Immunoprecipitation applications (Huang, S., et. al. (2005). J Biol Chem. 280(22):21183-93).

Flow Cytometry Analysis: A representative lot detected MR1 in flow cytometry applications (Huang, S., et. al. (2005). J Biol Chem. 280(22):21183-93; Gold, M.C., et. al. (2010). PLoS Biol. 8(6):e1000407; Huang, S., et. al. (2009). Proc Natl Acad Sci U S A. 106(20):8290-5).


Inhibits Activity/Function Analysis: A representative lot inhibited the activation of mucosal-associated invariant T (MAIT) cells by MR1 protein. (Huang, S., et. al. (2009). Proc Natl Acad Sci U S A. 106(20):8290-5).

Research Category
Inflammation & Immunology

Qualité

Evaluated by Flow Cytometry in MR1-transfected WT3 mouse embryonic fibroblasts.

Flow Cytometry Analysis: 1 µg of this antibody detected MR1 in one million MR1-transfected WT3 mouse embryonic fibroblasts.

Description de la cible

39.37 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.