MABE956

Anti-NFE2-related factor 1/Nrf1 Antibody, clone 17

clone 17, from rat

• Synonyme(s) : Nuclear factor erythroid 2-related factor 1, NF-E2-related factor 1, NFE2-related factor 1, Nuclear factor, erythroid derived 2, like 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 17, monoclonal
• Espèces réactives: mouse
• Technique(s): western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001123922
• Numéro d'accès UniProt: Q61985
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Nfe2(18022)

Description

Description générale

Nuclear factor erythroid 2-related factor 1 (NFE2-related factor 1/Nrf1), also known as NF-E2-related factor 1, NFE2-related factor 1, Nuclear factor erythroid derived 2 like 1, and encoded by the gene name Nfe2l1 and Nrf1, is a member of the bZIP family and the CNC subfamily. Isoform 3 of Nrf1 interacts with the extended kappa 3 site of the TNF alpha promoter after Fc gamma RIII stimulation and participates in the induction of this cytokine. Studies have suggested that isoform 1 of Nrf1 is either inactive or represses the transcriptional activation. Isoform 2 of Nrf1 has been shown to activate the expression of antioxidant response element (ARE) containing reporters and genes in cells, and may play a role in oxidative stress response. Other studies have suggested that isoform 1 also activates ARE-driven genes. Levels of Nrf1 isoform 2 in white fat, lung and liver are increased compared to isoform 1 (at protein level). Isoform 1 and isoform 2 of Nrf1 are widely expressed including kidney, brown fat, white fat, large intestine, small intestine, stomach, lung, brain and liver. Isoform 2 levels are elevated in brown fat and brain, but are reduced in liver compared to isoform 1 levels. Isoform 1 and isoform 2 are also expressed in mouse embryonic fibroblasts (MEF).

Immunogène

His-tagged recombinant protein corresponding to mouse NFE2-related factor 1/Nrf1.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-NFE2-related factor 1/Nrf1 antibody is validated for use in Western Blotting for the detection of NFE2-related factor 1/Nrf1.

Western Blotting Analysis: A representative lot detected NFE2-related factor 1/Nrf1 in MEF cells stimulated with MG132 (Tsuchiya, Y., et al. (2011) Mole. Cell Biol. 31(22):4500-4512).

Qualité

Evaluated by Western Blotting in MG132 treated NIH-3T3 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected NFE2-related factor 1/Nrf1 in 10 µg of MG132 treated NIH-3T3 cell lysate.

Description de la cible

~85 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forme physique

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable