MABE914

Anti-Mi-2β Antibody, clone 16G4

clone 16G4, from mouse

• Synonyme(s) : Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 16G4, monoclonal
• Espèces réactives: human
• Technique(s): ChIP: suitable (ChIP-seq); western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001264
• Numéro d'accès UniProt: Q14839
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CHD4(1108)

Description

Description générale

CHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.

Immunogène

Recombinant protein corresponding to Human Mi-2β.

Application

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was used for ChIP. See Zhang et al. (2012).

ChIP-seq: representative lot of this antibody was used for ChIP-seq. See Zhang et al. (2012).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

This Anti-Mi-2β Antibody, clone 16G4 is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of Mi-2β.

Qualité

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Mi-2β in a Jurkat cell lysate.

Description de la cible

~220 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2