MABE462

Anti-TET2 Antibody, clone hT2H 21F11

clone hT2H21F11, from mouse

• Synonyme(s) : Methylcytosine dioxygenase TET2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: hT2H21F11, monoclonal
• Espèces réactives: human, mouse
• Technique(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001120680
• Numéro d'accès UniProt: Q6N021
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TET2(54790)

Description

Description générale

Methylcytosine dioxygenase TET2 catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). The function of 5-hydroxymethylcytosine is currently unclear but it may influence chromatin structure, or act as an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD) or myeloproliferative neoplasms (MPN), and systemic mastocytosis. TET2 disorders also cause polycythemia vera (PV) and myelodysplastic syndrome (MDS).

Spécificité

This antibody recognizes the N-terminus of TET2

Immunogène

Recombinant protein corresponding to the N-terminus of human TET2.

Application

Detect Tet2 using this mouse monoclonal antibody, Anti-TET2 Antibody, clone hT2H 21F11 validated for use in Chromatin IP (ChIP), western blotting & IP.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected TET2 in 10 µg of TF-1 cell lysate.
Immunoprecipitation Analysis: 5 µg of a representative lot immunoprecipitated TET2 in 250 µg of HL60 RIPA cell lysate.

Qualité

Evaluated by Western Blotting in MOLT-4 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected TET2 in 10 µg of MOLT-4 cell lysate.

Description de la cible

~ 260 kDa observed. The calculated molecular weight of this protein is 224 kDa, but can be observed at ~260 kDa

Forme physique

Format: Purified

Remarque sur l'analyse

Control
MOLT-4 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable