MABE132

Anti-MyoD Antibody, clone 5F11

clone 5F11, from rat

• Synonyme(s) : Myoblast determination protein 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rat
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 5F11, monoclonal
• Espèces réactives: mouse
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_034996
• Numéro d'accès UniProt: P10085
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Myod1(17927)

Description

Description générale

Myoblast determination protein 1 (MyoD1) is a myogenic transcription factor required for the growth, repair, and regeneration of muscle cells. It induces differentiation of myoblast cells into multinucleated myotubules, activating genes and proteins involved in cell cycle arrest, and differentiation. MyoD1 may also control proliferation and homeostasis during embryogenesis, in the absence of Myf5.

Immunogène

GST-tagged recombinant protein corresponding to mouse MyoD.

Application

Detect MyoD using this Anti-MyoD Antibody, clone 5F11 validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A representative lot detected MyoD in C2C12 cells.

Western Blot Analysis: A representative lot detected MyoD in C2C12 cells.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Qualité

Evaluated by Western Blot in C2C12 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected MyoD in 10 µg of C2C12 cell lysate.

Description de la cible

~45 kDa observed. An uncharacterized band at ~38 kDa may be observed in some cell lysates. The calculated molecular weight is 34 kDa; however, this protein is known to be glycosylated.

Liaison

Replaces: MABE514

Forme physique

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
C2C12 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable