MABD129

Anti-PRDM1 Antibody, clone 5E7

ascites fluid, clone 5E7, from mouse

• Synonyme(s) : PR domain zinc finger protein 1, BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1, PRDI-BF1, PRDI-binding factor 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 5E7, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: O75626
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PRDM1(639)

Description

Description générale

PRDM1/Blimp1 is a repressor of beta-interferon gene expression. In the nucleus, PRDM1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, PRDM1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade. The transcription of this gene is increased upon virus infection.

Immunogène

Purified recombinant fragment of human PRDM1 expressed in E. Coli.

Application

Detect PRDM1 using this mouse monoclonal antibody, Anti-PRDM1 Antibody & clone 5E7 validated for use in western blotting.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Qualité

Evaluated by Western Blotting in Raji, L1210, and TPH-1 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PRDM1 in Raji, L1210, and TPH-1 cell lysates.

Optimal working dilutions must be determined by end user.

Description de la cible

~88 kDa observed. Uncharacterized bands may appear in some lysate(s).

Forme physique

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
Raji, L1210, and TPH-1 cell lysates

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable