MABC606

Anti-RNF43 Antibody, clone 11C3.1

clone 11C3.1, from mouse

• Synonyme(s) : E3 ubiquitin-protein ligase RNF43, RING finger protein 43
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 11C3.1, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_001292473
• Numéro d'accès UniProt: Q68DV7
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... RNF43(54894)

Description

Description générale

E3 ubiquitin-protein ligase RNF43 (UniProt Q68DV7; also known as RING finger protein 43) is encoded by the RNF43 gene (Gene ID 54894) in human. RNF43 and the homologous ZNRF3 are cell-surface transmembrane E3 ubiquitin ligases upregulated upon Wnt/ -catenin signaling and act as potent Wnt signaling pathway negative feedback regulators by mediating ubiquitination of Frizzled (FZD) and LRP6 for lysosomal degradation. RNF43/ZNRF3 activity is counteracted by stem cell growth factor R-spondin that induces membrane ZNRF3/RNF43 clearance by binding ZNRF3/RNF43 and LGR4/LGR5. Physical interaction with Dishevelled (DVL) is essential for ZNRF3/RNF43 Wnt inhibitory activity, and DVL knockdown significantly increases cell surface levels of FZD and LRP6. In addition, RNF43 suppresses canonical and noncanonical Wnt signaling via distinct mechanisms. While interaction between the extracellular protease-associated (PA) domain and the cysteine-rich domain (CRD) of FZD and RNF43 intracellular RING finger domain is necessary for canonical Wnt/ -catenin signaling inhibition, interaction between FZD PDZ domain and RNF43 C-terminal cytoplasmic region is essential for suppressing noncanonical Wnt signaling. R-spondin overexpression through gene translocation and loss-of-function RNF43/ZNRF3 gene mutations are found in human cancers as means to escape from Wnt signaling negative feedback regulation. Human RNF43 is produced with a signal peptide (a.a. 1-23) sequence, which is posttranslationally removed to yiled the mature enzyme with an N-terminal extracellular domain (a.a. 24-197), a transmembrane domain (a.a. 198-218), and a large cytoplasmic region (a.a. 219-783) that contains an atypical RING-type zinc finger (a.a. 272-313) and a pro-rich (a.a. 569-760) region.

Spécificité

Clone 11C3.1 epitope lies within the cytoplasmic pro-rich region present in all four spliced isoforms of human RNF43 reported by UniProt (Q68DV7).

Immunogène

GST-tagged recombinant human RNF43 fragment from the cytoplasmic pro-rich region.

Application

Detect RNF43 using this mouse monoclonal Anti-RNF43, clone 11C3.1 Antibody, Cat. No. MABC606, validated for use in Western Blotting.

Research Category
Apoptosis & Cancer

Qualité

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RNF43 in 10 µg of HCT116 human colon carcinoma cell lysate.

Description de la cible

~95 kDa observed. Target band size appears larger than the calculated molecular weights of 83.20/85.72 kDa (isoform 1 mature/pro-form), 78.71/81.23 kDa (isoform 2 mature/pro-form), 72.11 kDa (isoform 3) 92.52/95.04 kDa (isoform 4 mature/pro-form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified.

Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable