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MAB4401

Sigma-Aldrich

Anti-Oct-4 Antibody, clone 10H11.2

clone 10H11.2, Chemicon®, from mouse

Synonyme(s) :

Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Forme d'anticorps

purified antibody

Clone

10H11.2, monoclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

Entrée

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

Isotype

IgG1

Numéro d'accès NCBI

NM_002701.4

Numéro d'accès UniProt

Q01860

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... POU5F1(5460)

Description générale

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
Purified monoclonal antibody for the detection of Human Oct-4.

Spécificité

Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.

Application

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
Flow Cytometry: A previous lot of this antibody was used in FC.

ELISA: A previous lot of this antibody was used in ELISA.

Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

Optimal working dilutions must be determined by the end user.

Qualité

Evaluated by western blot on mouse P1 brain lysates.

Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates

Description de la cible

44 kDa

Forme physique

Format: Purified

Remarque sur l'analyse

Control
Human embryonic stem cell lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

En option

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos.
Chen, CH; Chang, WF; Liu, CC; Su, HY; Shyue, SK; Cheng, WT; Chen, YE; Wu, SC; Du, F; Sung, LY; Xu, J
Reproductive Biomedicine Online null
Microporous membrane growth substrates for embryonic stem cell culture and differentiation.
Steven D Sheridan,Sonia Gil,Matthew Wilgo,Aldo Pitt
Methods in Cell Biology null
Characterization of differential properties of rabbit tendon stem cells and tenocytes.
Zhang, J; Wang, JH
BMC Musculoskelet. Disord. null
Efficient, high-throughput transfection of human embryonic stem cells.
Moore, JC; Atze, K; Yeung, PL; Toro-Ramos, AJ; Camarillo, C; Thompson, K; Ricupero et al.
Stem Cell Research & Therapy null
Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
Chen, CH; Xu, J; Chang, WF; Liu, CC; Su, HY; Chen, YE; Du, F; Sung, LY
Reproductive Biomedicine Online null
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