MAB353A4

Anti-Nestin Antibody, clone rat-401, Alexa Fluor^™ 488 conjugate

clone rat-401, from mouse, ALEXA FLUOR^™ 488

• Synonyme(s) : rat401, NES, Nbla00170, FLJ21841
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 488
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: rat-401, monoclonal
• Espèces réactives: rat, mouse
• Ne doit pas réagir avec: human
• Technique(s): immunocytochemistry: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NP_037119
• Numéro d'accès UniProt: P21263
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

Description

Description générale

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

Spécificité

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

Immunogène

Embryonic rat spinal cord

Application

Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).

Research Category
Stem Cell Research

Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Qualité

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

Description de la cible

The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

Forme physique

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Stockage et stabilité

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Remarque sur l'analyse

Control
Mouse cortical neural stem cells (SCR029)

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable