MAB2259

Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7

clone 1A7, from mouse

• Synonyme(s) : Cytochrome P450 46A1, cholesterol 24-hydroxylase, cytochrome P450, family 46, cytochrome P450, family 46, subfamily A, polypeptide 1, cytochrome P450, subfamily 46 (cholesterol 24-hydroxylase)
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 1A7, monoclonal
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), human (based on 100% sequence homology), rabbit (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_006659
• Numéro d'accès UniProt: Q9Y6A2
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CYP46A1(10858)

Description

Description générale

In enzymology, a cholesterol 24-hydroxylase (EC 1.14.13.98) is an enzyme that catalyzes the chemical reaction:
cholesterol + NADPH + H+ + O2 --> (24S)-24-hydroxycholesterol + NADP+ + H2O. Cholesterol 24-hydroxylase, a cytochrome P450 (CYP46A1), is expressed at 100-fold higher levels in the brain than in the liver. This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. Studies indicate that cholesterol 24-hydroxylase constitutes a major tissue-specific pathway for cholesterol turnover in the brain (Lund, 2003).

Spécificité

This antibody recognizes cholesterol-24 hydroxylase.

Immunogène

Epitope: unknown

His-tagged recombinant protein corresponding to human cholesterol-24 hydroxylase.

Application

Detect Cholesterol-24 Hydroxylase using this Anti-Cholesterol-24 Hydroxylase Antibody, clone1A7 validated for use in WB, IP, IC, IH.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Qualité

Evaluated by Western Blot in Cholesterol-24 hydroxylase mouse brain lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Cholesterol-24 hydroxylase on 10 µg of Cholesterol-24 hydroxylase mouse brain lysate.

Description de la cible

~ 54 kDa

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Mouse brain lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable