MAB1072

Anti-Erythropoietin Antibody, clone 1C8

clone 1C8, Chemicon^®, from mouse

• Synonyme(s) : erythropoietin, Epoetin, epoetin
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Clone: 1C8, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• Isotype: IgG2b
• Numéro d'accès NCBI: NM_000799.2
• Numéro d'accès UniProt: P01588
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Erythropoietin (Epo) is a glycoprotein consisting of one 165-amino acid single-chain polypeptide with two internal disulfide bonds. When fully glycosylated, the molecular weight of Epo is approximately 34 kDa. Normally produced by the liver and kidney, Epo is the principle regulator of red blood cell production by stimulating the division and differentiation of bone marrow erythroid progenitor cells in response to anemia and hypoxia.

Spécificité

Reacts with human Erythropoietin (Epo).

Immunogène

Recombinant human Epo.

Application

Detect Erythropoietin using this Anti-Erythropoietin Antibody, clone 1C8 validated for use in ELISA, IF & WB.

ELISA:
For the determination of Epo concentration in the range of 2-200 ng/mL. Use at 5 ug/mL to coat plate.

Immunosorbent preparation

Optimal working dilutions must be determined by the end user.

Research Category
Metabolism

Research Sub Category
Metabolic Hormones & Receptors

Qualité

Evaluated by Western Blot on Mouse kidney lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected glycosylated EPO on 10 µg of Mouse kidney lysates.

Description de la cible

34 kDa (glycosylated)

Forme physique

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2b in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Stockage et stabilité

Stable for 1 year at 2-8ºC from date of receipt.

Remarque sur l'analyse

Control
Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable