MAB1021
Anti-Tumor Necrosis Factor-α Antibody, clone 2C8
clone 2C8, Chemicon®, from mouse
Synonyme(s) :
TNF-alpha
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Clone
2C8, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
ELISA: suitable
immunohistochemistry: suitable
neutralization: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TNF(7124)
Spécificité
Human tumor necrosis factor-a (TNF-a)
No cross-reactivity observed with rat neuronal tissue.
Immunogène
Human recombinant tumor necrosis factor α.
Application
Detect Tumor Necrosis Factor-α using this Anti-Tumor Necrosis Factor-α Antibody, clone 2C8 validated for use in ELISA, IH, NEUT.
Inhibition Assays – Inhibition of biological activity of recombinant and natural TNF-α.
Detection of TNF-α in two-site immunoassay.
Immunohistochemistry detected TNF-α.
TNF-α Neutralization Assay
1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.
2. Incubate overnight at 37°C in humidified 5% CO2 incubator.
3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL
4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.
5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.
6. Incubate 24 hours at 37°C, 5% CO2.
7. Score wells for cytotoxicity.
Optimal working dilutions must be determined by the end user.
Detection of TNF-α in two-site immunoassay.
Immunohistochemistry detected TNF-α.
TNF-α Neutralization Assay
1. Plate 2 x 104 murine L929B cells into individual wells of 96-well (flat bottomed) microtiter plate.
2. Incubate overnight at 37°C in humidified 5% CO2 incubator.
3. The next day, prepare serial dilutions of anti-TNF (human) in medium containing 2 μg/mL of actinomycin D, for a final volume of 50 μL
4. Add 50 μL of TNF (10 ng/mL final concentration) to each antibody dilution and incubate for 1 hour at 37°C.
5. Add 100 μL of the antibody/antigen mixture to the pre-seeded L929B cells.
6. Incubate 24 hours at 37°C, 5% CO2.
7. Score wells for cytotoxicity.
Optimal working dilutions must be determined by the end user.
Liaison
Replaces: 04-1114
Forme physique
Format: Purified
Stockage et stabilité
Maintain at 2-8°C in undiluted aliquots for up to one year.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Propionibacterium acnes stimulates pro-matrix metalloproteinase-2 expression through tumor necrosis factor-alpha in human dermal fibroblasts.
Jee-Young Choi,Mei Shan Piao,Jee-Bum Lee,Jong Seok Oh,In-Gyu Kim,Seung-Chul Lee
The Journal of Investigative Dermatology null
A Klegeris et al.
Neuroscience letters, 313(1-2), 41-44 (2001-10-31)
We measured the secretion of interleukin (IL)1beta, IL-6 and tumor necrosis factor-alpha (TNF-alpha) from human monocytic (THP-1), astrocytic (U-373 MG) and neuronal (SH-SY5Y) cell lines alone and in co-culture, with and without stimulation by a combination of lipopolysaccharide (LPS) plus
Zifeng Liu et al.
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