MAB1012-I

Anti-Alkaline Phosphatase Antibody, E. coli

• Synonyme(s) : EC:3.1.3.1, JW0374, alkaline phosphomonoesterase, b0383, glycerophosphatase, phosphomonoesterase
• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: N/A, monoclonal
• Poids mol.: calculated mol wt 49.44 kDa; observed mol wt ~50 kDa
• Produit purifié par: using protein G
• Espèces réactives: E. coli
• Conditionnement: antibody small pack of 100
• Technique(s): western blot: suitable
• Isotype: IgG2bκ
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_414917
• Numéro d'accès UniProt: P00634
• Température de stockage: 2-8°C

Description

Spécificité

This mouse recombinant monoclonal antibody detects Alkaline Phosphatase from E. coli.

Immunogène

Purified Alkaline Phosphatase from E. coli.

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from E. coli.

Western Blotting Analysis: A 1:1,000 Dilution of this antibody detected Alkaline Phosphatase in E. coli lysate.

Tested Applications

Western Blotting Analysis: A representative lot detected Alkaline Phosphatase in Western Blotting application (Yogev, D., et al. (1991). J Bacteriol. 173(6):2035-44; Taw, M.N., et al. (2022). Sci Rep. 12(1):14862).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Alkaline phosphatase (UniProt: P00634; also known as EC:3.1.3.1, APase) is encoded by the phoA (also known as b0383, JW0374) gene (Gene ID: 945041) in E. coli. Alkaline phosphatase is a dimeric, non-glycosylated metalloenzyme, requiring two zinc ions (Zn²⁺) and one magnesium ion (Mg²⁺) per subunit for its activity and structural stability. The active site of Alkaline phosphatase includes a serine residue that plays a crucial role in its catalytic mechanism. The primary function of E. coli Alkaline phosphatase is to catalyze the hydrolysis of phosphate monoesters, resulting in the release of an inorganic phosphate and an alcohol. The enzyme is involved in various processes, including phosphate metabolism and regulation. Alkaline phosphatase is also known for its ability to transfer phosphate groups to acceptor molecules, which is key in its role in transphosphorylation reactions. Alkaline Phosphatase has a wide range of applications in molecular biology. It is used as a reporter enzyme in various assays, including ELISA and Western blotting. During molecular cloning, it is commonly used to dephosphorylate the 5′ ends of DNA fragments to prevent self-ligation during cloning procedures.

Forme physique

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable