MAB1012

Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only

ascites fluid, Chemicon^®

• Synonyme(s) : AP, Alk. Phos.
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: monoclonal
• Espèces réactives: E. coli
• Fabricant/nom de marque: Chemicon^®
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2a
• Numéro d'accès UniProt: P00634
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: Escherichia coli ... PhoA(945041)

Description

Spécificité

Alkaline phosphatase (AP). MAB1012 has a high affinity and recognizes an AP determinant resistant to denaturation by SDS-PAGE. It is therefore ideally suited for sensitive and specific detection of AP fusion proteins by Western blot analysis of E. coli transformants expressing fusion products.

Immunogène

Epitope: E. coli, bacterial only

F201C1B

Application

Anti-Alkaline Phosphatase Antibody, E. coli, bacterial only detects level of Alkaline Phosphatase & has been published & validated for use in IP, WB & IC.

Western blot at 1:5,000. 42-45kDa on SDS-PAGE,reducing gels for natural E. coli alkaline phosphatase monomer. Alkaline phosphatase fusion proteins will vary depending upon the target fusion protein.

Immunocytochemistry: reacts with E.coli. AP fusion protein targets in acetone fixed cell preparations. 1:4000, other fixatives or conditions untested.

ASSAY:

Preparation of E. coli TnphoA transformants: E. coli strain CC118 was transformed with plasmid pGEM-3Z containing TnphoA insertional mutations in the p101 gene of Mycoplasma hyorhinis, which encodes a protein with a typical N-terminal prokaryotic single peptide (Yogev et al. 1991).

Identification of fusion protein with MAB1012 transformants: Transformants are grown in 2XYT medium to OD600=0.6. Cells were centrifuged 3 minutes at 10,000 x g, suspended in SDS-PAGE sample buffer, heated at 100°C for 5 minutes, frozen and thawed and centrifuged as above at room temperature to remove insoluble material. The sample is applied at 9% to a SDS-PAGE gel, and Western immunoblot is performed as described (Yogev et al. 1991).

Immunoprecipitation: 5μL of antibody per 500μL of lysate in RIPA or 0.5% triton X-100 solutions.

Optimal working dilutions must be determined by end user.

Forme physique

Ascites. Contains no preservative.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable