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Merck
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Key Documents

IP02

Millipore

Protein A Agarose Suspension

Protein A Agarose Suspension designed for immunoprecipitation applications

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About This Item

Code UNSPSC :
41116133
Nomenclature NACRES :
NA.56

Forme

slurry (Liquid)

Contient

≤0.1% sodium azide as preservative

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

do not freeze

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG2a and IgG2b and rabbit IgG.
Protein A conjugated to agarose.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In PBS.

Autres remarques

Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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American journal of physiology. Cell physiology, 314(2), C177-C190 (2017-11-03)
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Biochemical and biophysical research communications, 368(2), 279-284 (2008-01-29)
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eLife, 5 (2016-02-18)
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Vineet Kumar Maurya et al.
Journal of ovarian research, 7, 32-32 (2014-03-19)
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