FCMAB103AP

Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate

clone Ki-S5, Milli-Mark^®, from mouse

• Synonyme(s) : MKI67 ; ; KIA ; ; Ki-67
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: Allophycocyanin conjugate
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: Ki-S5, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Milli-Mark^®
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NM_002417.3
• Numéro d'accès UniProt: P46013
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MKI67(4288)

Description

Description générale

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin′s lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer.

Spécificité

Antibody recognizes Ki67.

Immunogène

Nuclear protein preparation from the human cell line U937.50

Application

Detect Ki67 using this Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate validated for use in FC, IC, IH, FC, IH(P).

Research Category
Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

Qualité

Evaluated by Flow Cytometry with Jurkat cells.

Description de la cible

345 & 395 kDa observed

Forme physique

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Allophycocyanin (APC) in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Stockage et stabilité

Maintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.

Remarque sur l'analyse

Control
Stimulated Jurkat Cells

Informations légales

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable