ABT98

Anti-Kinectin Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Kinectin, CG-1 antigen, Kinesin receptor
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rhesus macaque (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_001072989
• Numéro d'accès UniProt: Q86UP2
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KTN1(3895)

Description

Description générale

Organelle transport is facilitated by molecular motors, such as kinesin and dynein, which interact with microtubules to transport cargo in an ATP-dependent manner. The shuttling of organelles is also dependent on kinectin, a transmembrane ER protein that associates with kinesin and stimulates its ATPase activity. Kinesin-kinectin dynamics have also been linked to focal adhesion growth. In addition, kinectin has been shown to activate the RhoG GTPase pathway which involves Rac1 and Cdc42 signaling. Kinectin is expressed in testis, ovary, spleen, thymus, prostate, colon, and small intestine.

Spécificité

Other homologies: Mouse (82% Mouse sequence homology) and Rat (79% sequence homology).

This antibody recognizes the lumenal region of Kinectin.

Immunogène

Epitope: Lumenal region

KLH-conjugated linear peptide corresponding to the lumenal region of human Kinectin.

Application

Detect Kinectin using this Anti-Kinectin Antibody validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Kinectin in HeLa and A431 cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Qualité

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Kinectin in 10 µg of human testis tissue lysate.

Description de la cible

~158 kDa observed. Uniprot describes 3 isoforms at ~149, ~150, and ~156 kDa

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Human testis tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable