ABT1493-AF488

Anti-Cofilin-1 Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• Synonyme(s) : Cofilin-1, 18 kDa phosphoprotein, Cofilin, non-muscle isoform, p18
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 488
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, mouse
• Réactivité de l'espèce (prédite par homologie): porcine (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable
• Numéro d'accès NCBI: NP_005498
• Numéro d'accès UniProt: P23528
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CFL1(1072)

Description

Description générale

Cofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.

Spécificité

This Alexa Fluor^™ 488-conjugated polyclonal antibody targets cofilin-1 N-terminal end sequence conserved among bovine, human, mouse, porcine, and rat species.

Immunogène

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-termnal end sequence of human cofilin-1.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cofilin-1 in NIH/3T3 cells.

The unconjugated antibody (Cat. No. ABT1493) is also available for immunocytochemistry, immunoprecipitation, and Western blotting applications.

Research Category
Cell Structure

This rabbit polyclonal Anti-Cofilin-1 Antibody, Alexa Fluor^™ 488 Conjugate, Cat. No. ABT1493-AF488 is validated for use in Immunocytochemistry for the detection of cofilin-1.

Qualité

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cofilin-1 in HeLa cells.

Description de la cible

~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed).

Forme physique

Affinity purified.

Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable