ABS233
Anti-PI3 Kinase p85 Antibody, N-SH2 domain
from rabbit, purified by affinity chromatography
Synonyme(s) :
Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-al
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
human, rat
Réactivité de l'espèce (prédite par homologie)
mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)
Technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
bovine ... Pik3R1(282307)
human ... PIK3R1(5295)
mouse ... Pik3R1(18708)
rat ... Pik3R1(25513)
rhesus monkey ... Pik3R1(698996)
Description générale
Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt Q63787; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the Pik3r1 gene (Gene ID 25513) in rat species. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in Pik3r1 gene are known to be associated with insulin resistance.
Spécificité
This polyclonal antibody targets the first SH2 domain region common to human (isoform 1, AS53, p46, p85I), mouse, and rat (isoform p85-alpha, p55-alpha, and p50-alpha) spliced isoforms of PI3 kinase regulatory subunit alpha reported by UniProt (P27986, P26450, Q63787).
Immunogène
Epitope: First SH2 domain.
GST-tagged recombinant fragment corresponding to the first SH2 domain of rat PI3 kinase regulatory subunit alpha.
Application
Detect PI3 Kinase regulatory subunit alpha using this rabbit polyclonal Anti-PI3 Kinase p85, N-SH2 domain, Cat. No. ABS233, validated for use in Immunoprecipitation and Western Blotting.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 200 µg of rat brain tissue lysate.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 500 µg of Jurkat cell lysate.
Western Blotting Analysis: 2 µg/mL from a representative lot detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of Jurkat cell lysate.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 500 µg of Jurkat cell lysate.
Western Blotting Analysis: 2 µg/mL from a representative lot detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of Jurkat cell lysate.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Qualité
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of rat brain tissue lysate.
Description de la cible
~85/50 kDa observed. 83.47/53.49/49.97/84.47 kDa (human isoform 1/AS53/p46/p85I; UniProt P27986), 83.39 kDa (mouse; UniProt P26450), 83.40/53.39/50.00 kDa (rat p85-alpha/p55-alpha/p50-alpha isoforms; UniProt Q63787). Uncharacterized bands may be observed in some lysate(s).
Liaison
Replaces: 06-496
Forme physique
Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Remarque sur l'analyse
Control
Jurkat cell lysate
Jurkat cell lysate
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Yu-Jie Hu et al.
F1000Research, 2, 279-279 (2013-01-01)
Protein arginine methylation is a common posttranslational modification that has been implicated in numerous biological processes including gene expression. The mammalian genome encodes nine protein arginine methyltransferases (Prmts) that catalyze monomethylation, asymmetric dimethylation, and symmetric dimethylation on arginine residues. Protein
Yu-Jie Hu et al.
Nucleic acids research, 43(16), 7790-7804 (2015-06-29)
Jumonji domain-containing protein 6 (JMJD6) is a nuclear protein involved in histone modification, transcription and RNA processing. Although JMJD6 is crucial for tissue development, the link between its molecular functions and its roles in any given differentiation process is unknown.
Teresita Padilla-Benavides et al.
International journal of molecular sciences, 21(3) (2020-02-06)
Brg1 (Brahma-related gene 1) is one of two mutually exclusive ATPases that can act as the catalytic subunit of mammalian SWI/SNF (mSWI/SfigureNF) chromatin remodeling enzymes that facilitate utilization of the DNA in eukaryotic cells. Brg1 is a phospho-protein, and its
Timothy J Thauland et al.
Frontiers in immunology, 10, 753-753 (2019-04-30)
Gain-of-function variants in p110δ, the catalytic subunit of phosphatidylinositol 3-kinase (PI3K) expressed in lymphocytes, cause activated PI3-kinase δ syndrome (APDS), a primary immunodeficiency that is characterized by recurrent infections, viremia, lymphadenopathy, and autoimmunity. The mechanism of autoimmunity in APDS has
Jonathon N Winnay et al.
The Journal of clinical investigation, 126(4), 1401-1412 (2016-03-15)
The phosphatidylinositol 3-kinase (PI3K) signaling pathway is central to the action of insulin and many growth factors. Heterozygous mutations in the gene encoding the p85α regulatory subunit of PI3K (PIK3R1) have been identified in patients with SHORT syndrome - a
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