ABS1020

Anti-Acylation Stimulating Protein Antibody

from rabbit

• Synonyme(s) : Complement C3, C3adesArg, C3, PZP-like alpha-2-macroglobulin domain-containing protein 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Technique(s): ELISA: suitable; flow cytometry: suitable; neutralization: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_000055
• Numéro d'accès UniProt: P01024
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... C3(718)

Description

Description générale

The protein called Acylation stimulating protein (ASP) is a cleavage fragment protein from the Complement C3 gene. C3 plays a central role in the activation of the complement system. C3’s processing by C3 convertase is the central reaction in both classical and alternative complement pathways. ASP acts as an adipogenic hormone that stimulates triglyceride synthesis and glucose transport in adipocytes, and thus it helps regulate fat storage and triglyceride synthesis. ASP stimulates triglyceride synthesis via PLC, MAPK and AKT signaling pathways and ASP promotes the phosphorylation and internalization and recycling of Complement factor C5AR2. ASP is expressed in adipocytes and released into the plasma during both the fasting and postprandial periods. Increased levels of C3 and its cleavage product ASP are associated with obesity, diabetes and coronary heart disease. Short-term endurance training reduces baseline ASP levels and subsequently fat storage.

Immunogène

recombinant protein corresponding to Human Acylation Stimulating Protein.

Application

Flow Cytometry Analysis: 10 µg/mL of this antibody blocked ASP binding to HEK-hCL52 cells (Wei, C,. et al, Am J Physiol Endocrinol Metab 293:E1482-E1491, 2007)
Neutralizing Assay Analysis: A representative lot of this antibody demonstrated neutralizing effect of ASP stimulation of TG synthesis and glucose transport in HEK-hC5L2 cells,
3T3-L1 preadipocytes, and 3T3-L1 adipocytes (Wei, C,. et al, Am J Physiol Endocrinol Metab 293:E1482-E1491, 2007)
ELISA Analysis: 0.5 µg/mL of this antibody detected ASP (PEG precipitated) in a sandwich ELISA format (Saleh, J,. et al, J. Lipid Res. 1998. 39: 884–891)

This Anti-Acylation Stimulating Protein Antibody is validated for use in Western Blotting and Flow Cytometry and Neutralizing and ELISA for the detection of Acylation Stimulating Protein.

Qualité

Evaluated by Western Blotting in HEK293 expressing ASP cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acylation Stimulating Protein in 10 µg of HEK293 expressing ASP cell lysate.

Description de la cible

~ 16/10 kDa observed, 10 kDa indicates native ASP, 16 kDa indicates HIS-Tag ASP. HIS-Tag was added for purification purposes. We have found no difference in Activity (measured by TGS, Fatty Acid uptake, etc) between rASP with/without the tag. Further, the ELISA was successful in measuring both.

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable